10803 Ccdc48 Status: Cancelled
| Gene | Ccdc48 |
| Chromosome | 6 |
| Deletion Start | 87,730,878 |
| Deletion End | 87,733,016 |
| Deletion Size | 2,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,731,804 | 87,731,828 | GCGAAACTTGTTGAATCCCTCACAG |
| TUP | 87,731,830 | 87,731,853 | CAGGCCTCTAGCCCAGGTGAGAAA |
| TUR | 87,731,869 | 87,731,888 | AGTGAGCCTGGCCCTCATTG |
| TDF | 87,732,018 | 87,732,040 | GGAAGAGGACCTGCGTTACATTG |
| TDP | 87,732,042 | 87,732,066 | TGTCTCCTGTCTCCTAGTTGCTGCA |
| TDR | 87,732,081 | 87,732,101 | AACCACGAAGGCCACTTTGAC |
PCR Primers
| Primer | Sequence |
| SU | TGGTGACAATAGCGGAAAGG |
| SD | CAGGATCAAACTGAAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
