10806 Abra Status: Cancelled
| Gene | Abra |
| Chromosome | 15 |
| Deletion Start | 41,869,665 |
| Deletion End | 41,865,819 |
| Deletion Size | 3,847 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,869,588 | 41,869,608 | CGCACAGCAACCCTGGTTATC |
| TUP | 41,869,565 | 41,869,587 | AATTTGGCCCGAGGTTGGCAGCA |
| TUR | 41,869,533 | 41,869,554 | GGGCCTGTTTGGTACTGTTCTC |
| TDF | 41,866,275 | 41,866,297 | GTGGACAACTTGAAAGGGAGGTG |
| TDP | 41,866,250 | 41,866,273 | CAGCAGTGGGCCGATGAACACGTC |
| TDR | 41,866,229 | 41,866,249 | GGGATTGAGCTTCTGGGACTG |
PCR Primers
| Primer | Sequence |
| SU | ACCAGAAATGTCCCTCTGCT |
| SD | CCATTCTGAGCTGGTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 460 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
