10807 Acrv1 Status: Cancelled
| Gene | Acrv1 |
| Chromosome | 9 |
| Deletion Start | 36,693,315 |
| Deletion End | 36,698,631 |
| Deletion Size | 5,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,693,686 | 36,693,704 | GCACTGTGGCACCTGTAAC |
| TUP | 36,693,705 | 36,693,733 | TCCTACAGTCAAGAGGTAGAAGTGGGAGA |
| TUR | 36,693,760 | 36,693,778 | GCAACAAGGTCTGGCTGTG |
| TDF | 36,698,451 | 36,698,471 | AAGGTTGGAAGCAGCTTACTG |
| TDP | 36,698,474 | 36,698,500 | ACTGCCAATCTGTCTCTTTCAGGTGGA |
| TDR | 36,698,509 | 36,698,529 | TCACACCCTTGAACCATGAAC |
PCR Primers
| Primer | Sequence |
| SU | GACCCTCTGCAAAGAAGTGC |
| SD | CACTTTGTTCATGGACAGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 460 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 385 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
