10809 Actrt1 Status: Cancelled
| Gene | Actrt1 |
| Chromosome | X |
| Deletion Start | 46,329,113 |
| Deletion End | 46,330,244 |
| Deletion Size | 1,132 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,329,502 | 46,329,521 | TAACGTGCCTGCCTTGTATC |
| TUP | 46,329,524 | 46,329,545 | TGCAACCACGCAGTAGGAGCCT |
| TUR | 46,329,606 | 46,329,626 | AGCCTTCATAGACGGGAACAG |
| TDF | 46,330,093 | 46,330,116 | CAAGATCACAGCTTCCTCAGACAG |
| TDP | 46,330,121 | 46,330,148 | TATTCGGCTTGGATTGGTGGATCTGTCA |
| TDR | 46,330,174 | 46,330,192 | CTCAGCAGTGACCCACATC |
PCR Primers
| Primer | Sequence |
| SU | GTGTTGGCTCAGAGACATCC |
| SD | CAAATAAAATAAAATTACTTTGGGGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 379 bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 257 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
