10810 Aim2 Status: ES cell colonies screened / QC no positives
| Gene | Aim2 |
| Chromosome | 1 |
| Deletion Start | 173,462,167 |
| Deletion End | 173,467,673 |
| Deletion Size | 5,507 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 173,462,746 | 173,462,769 | CGCCTTGTAGCTTCTGAAGCATAG |
| TUP | 173,462,771 | 173,462,796 | CACAGGCATCTTCACCTCTGTGTTGC |
| TUR | 173,462,829 | 173,462,847 | TGCTCAACCCAACAGTGAC |
| TDF | 173,467,079 | 173,467,102 | TTCATGGCAAAGAGACCTGTTATG |
| TDP | 173,467,108 | 173,467,131 | TATATGCACAGCCCAGGGAGTGGC |
| TDR | 173,467,150 | 173,467,174 | AGGAGGCCACACCTAATTCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGTTGTCACGGTGCTGAAAG |
| SD | CTACAACTATACTGATCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
