Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

10812 Alg10b Status: ES cell colonies screened / QC positives

Gene	Alg10b
Chromosome	15
Deletion Start	90,224,424
Deletion End	90,228,474
Deletion Size	4,051
Genome Build	38

MGI ID	MGI:2146159
NCBI Gene ID	380959
BAC ID	324P14
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	90,224,647	90,224,667	CCAACCTGTGCCTTTCCTCTC
TUP	90,224,668	90,224,690	CCCGTCAGCCCTCCCAGACTTAA
TUR	90,224,694	90,224,713	CACTAGGCAGCTCCCTTCAC
TDF	90,227,671	90,227,692	GGTGTACGCCATGTCCCTAAAG
TDP	90,227,697	90,227,723	TGAGAATGCTCTTCCTGTTGACCTGGC
TDR	90,227,738	90,227,759	CAAAGGCAAAGAACGCCAACAG

PCR Primers

Primer	Sequence
SU	GATTGACAGAGAAACCGTCC
SD	GTGGGTACGACAATATCTGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	353 bp	-	+	+
TUF	TUR	67 bp	+	+	-
TDF	TDR	89 bp	+	+	-
NeoFwd	SD	340 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
10812A-D4	VGB6	C57BL/6NTac
10812A-F9	VGB6	C57BL/6NTac
10812A-A9	VGB6	C57BL/6NTac
10812A-H1	VGB6	C57BL/6NTac