10814 1500015O10Rik Status: ES cell colonies screened / QC no positives
| Gene | 1500015O10Rik |
| Chromosome | 1 |
| Deletion Start | 43,742,460 |
| Deletion End | 43,730,888 |
| Deletion Size | 11,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,731,705 | 43,731,724 | CTGGGAGGCAGTAGGGTGAC |
| TUP | 43,731,734 | 43,731,762 | ACTGCCAAGAGAACAGTAGCTGGAAGACA |
| TUR | 43,731,794 | 43,731,813 | CCCTTCGCACTGGGACAAAC |
| TDF | 43,741,733 | 43,741,755 | TTTGAGCATCTTCCAGTGTCTTC |
| TDP | 43,741,762 | 43,741,788 | CCTGACACCCACAGCCCACTTCACAAT |
| TDR | 43,741,801 | 43,741,823 | TGCATGTTCTTGGTCTGAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | CCAACATTCTCTGTCCAGGC |
| SD | GCATCCCAGAGCAGAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
