10816 Apon Status: ES cell colonies screened / QC positives
| Gene | Apon |
| Chromosome | 10 |
| Deletion Start | 127,691,514 |
| Deletion End | 127,692,284 |
| Deletion Size | 771 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,691,523 | 127,691,543 | GCATTGCTTCTTGGCTGTATC |
| TUP | 127,691,552 | 127,691,571 | CCCGGTGACCGCCTTTCCGT |
| TUR | 127,691,603 | 127,691,624 | TGATGTGGGTTCAGTTCTTGTG |
| TDF | 127,692,056 | 127,692,077 | GGCCTCTGTAGTCAATGTCACC |
| TDP | 127,692,078 | 127,692,103 | CAGCACTGTACCATGGAGTCTTGGGA |
| TDR | 127,692,114 | 127,692,134 | CATCAGCCTCCTGGCTACTTC |
PCR Primers
| Primer | Sequence |
| SU | GTTAACCTCTGGAAGGTAGC |
| SD | AGGCCCAGAATTCAGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10816C-A8 | VGB6 | C57BL/6NTac | |
| 10816C-B5 | VGB6 | C57BL/6NTac | |
| 10816C-B9 | VGB6 | C57BL/6NTac | |
| 10816C-D2 | VGB6 | C57BL/6NTac | |
| 10816C-E11 | VGB6 | C57BL/6NTac | |
| 10816C-G4 | VGB6 | C57BL/6NTac | |
