| Gene | Aqp12 |
| Chromosome | 1 |
| Deletion Start | 93,006,386 |
| Deletion End | 93,012,209 |
| Deletion Size | 5,824 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,006,941 | 93,006,958 | TGCAGCACAGCCTTCTTG |
| TUP | 93,006,961 | 93,006,980 | TACAGGGTGCCTGCCCTGGC |
| TUR | 93,006,992 | 93,007,013 | TTACCTGTGTAGGCCATGAGAG |
| TDF | 93,011,865 | 93,011,884 | GGAGAGGTGCAGCCAGAAAG |
| TDP | 93,011,885 | 93,011,907 | AGCCAGACCCGCACTCAGGTAGG |
| TDR | 93,011,919 | 93,011,940 | GAACGTAAACTGTCACCCAGGT |
PCR Primers
| Primer | Sequence |
| SU | TGGGGAAGACGCCAAGCAG |
| SD | GCCAGGTTATCACGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 323 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 288 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10817A-C4 | VGB6 | C57BL/6NTac | |
| 10817A-C11 | VGB6 | C57BL/6NTac | |
| 10817A-E10 | VGB6 | C57BL/6NTac | |
| 10817A-F7 | VGB6 | C57BL/6NTac | |
