10817 Aqp12 Status: ES cell colonies screened / QC positives
| Gene | Aqp12 |
| Chromosome | 1 |
| Deletion Start | 94,902,963 |
| Deletion End | 94,908,786 |
| Deletion Size | 5,824 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,903,518 | 94,903,535 | TGCAGCACAGCCTTCTTG |
| TUP | 94,903,538 | 94,903,557 | TACAGGGTGCCTGCCCTGGC |
| TUR | 94,903,569 | 94,903,590 | TTACCTGTGTAGGCCATGAGAG |
| TDF | 94,908,442 | 94,908,461 | GGAGAGGTGCAGCCAGAAAG |
| TDP | 94,908,462 | 94,908,484 | AGCCAGACCCGCACTCAGGTAGG |
| TDR | 94,908,496 | 94,908,517 | GAACGTAAACTGTCACCCAGGT |
PCR Primers
| Primer | Sequence |
| SU | TGGGGAAGACGCCAAGCAG |
| SD | GCCAGGTTATCACGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 323 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 288 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10817A-C4 | VGB6 | C57BL/6NTac | |
| 10817A-C11 | VGB6 | C57BL/6NTac | |
| 10817A-E10 | VGB6 | C57BL/6NTac | |
| 10817A-F7 | VGB6 | C57BL/6NTac | |
