10819   Naa11     Status: Cancelled

GeneNaa11
Chromosome5
Deletion Start97,392,294
Deletion End97,391,621
Deletion Size674
Genome Build38
MGI IDMGI:2141314
NCBI Gene ID97243
BAC ID350E9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,392,22097,392,237TGCCTGCCGGAGAACTAC
TUP97,392,18397,392,212AGTACTACTTCTACCATGGCCTGTCGTGGC
TUR97,392,14497,392,162TCTTGCCGTCCTCATCCTC
TDF97,391,92697,391,947GGCCCTGCACCTGTATTCTAAC
TDP97,391,89797,391,921TCAACTTCCAGGTCAGTGAGGTGGA
TDR97,391,85697,391,876TCATGGCGTAAGCATCTTCAC

PCR Primers

PrimerSequence
SUCAGGTATGCAATGAGTCCCA
SDTCCCATTCTGCATTTCGCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev349 bp-++
TUFTUR94 bp++-
TDFTDR92 bp++-
NeoFwdSD349 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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