10821 Arl4c Status: ES cell colonies screened / QC no positives
| Gene | Arl4c |
| Chromosome | 1 |
| Deletion Start | 88,701,661 |
| Deletion End | 88,701,064 |
| Deletion Size | 598 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,701,599 | 88,701,617 | CGTTATGCTGGGCTTGGAC |
| TUP | 88,701,579 | 88,701,598 | TCGGCCGGCAAGACTACGGT |
| TUR | 88,701,556 | 88,701,577 | CGTTGAACTTGAGCCGGTAGAG |
| TDF | 88,701,311 | 88,701,331 | ACCAAGTTTGCGGAGAACCAG |
| TDP | 88,701,285 | 88,701,308 | CACACCGCTCCTGGTTATCGCCAA |
| TDR | 88,701,228 | 88,701,248 | AGCGCCAGTTGCTTCTCAATC |
PCR Primers
| Primer | Sequence |
| SU | GCGCGAGAGACAAAGCCTC |
| SD | ACAAAAGGTCCTCTGAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 359 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
