10822 Arl9 Status: ES cell colonies screened / QC positives
| Gene | Arl9 |
| Chromosome | 5 |
| Deletion Start | 77,433,083 |
| Deletion End | 77,438,501 |
| Deletion Size | 5,419 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,433,227 | 77,433,247 | GGCCTTGTCCATACCATAGTG |
| TUP | 77,433,249 | 77,433,276 | TGACTCAGAAAGGCACTGCAACACTTCG |
| TUR | 77,433,287 | 77,433,307 | TCATAACCCTGCCCTAACCTC |
| TDF | 77,438,092 | 77,438,109 | AGCAGTGGAGGAGGGAAC |
| TDP | 77,438,110 | 77,438,135 | TGCAAAGACTTAGACGCTCCAGTACC |
| TDR | 77,438,138 | 77,438,157 | GAGTGCTACACAGCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGACTCTTAGAGCTACC |
| SD | CCTGTCTCAAATAGAGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 414 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10822A-C4 | VGB6 | C57BL/6NTac | |
| 10822A-D11 | VGB6 | C57BL/6NTac | |
| 10822A-F9 | VGB6 | C57BL/6NTac | |
