| Gene | Mettl13 |
| Chromosome | 1 |
| Deletion Start | 162,548,253 |
| Deletion End | 162,534,266 |
| Deletion Size | 13,988 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 162,547,783 | 162,547,801 | TCACTGCCGAGTGCTCATC |
| TUP | 162,547,758 | 162,547,779 | TTTCAGGCTTTGTGCCCAGCCC |
| TUR | 162,547,733 | 162,547,752 | GTGGCTTCCTGCTCCTGTAG |
| TDF | 162,534,504 | 162,534,523 | GGGACGTGAGGTTAAAGGAC |
| TDP | 162,534,481 | 162,534,503 | TCAGTGCTGGCTGGACTCAAGGC |
| TDR | 162,534,454 | 162,534,472 | CGCCGGACATAGAGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTGAGACTCCTGCTCCTG |
| SD | TGCTGCCTCCCATCCCAAA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10824B-B10 | VGB6 | C57BL/6NTac | |
| 10824B-C9 | VGB6 | C57BL/6NTac | |
| 10824B-D5 | VGB6 | C57BL/6NTac | |
| 10824B-F3 | VGB6 | C57BL/6NTac | |
| 10824B-C6 | VGB6 | C57BL/6NTac | |
