10828 Bhlhe41 Status: Cancelled
| Gene | Bhlhe41 |
| Chromosome | 6 |
| Deletion Start | 145,865,264 |
| Deletion End | 145,862,830 |
| Deletion Size | 2,435 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,865,001 | 145,865,022 | GGAGCTTGAAGCGAGACGATAC |
| TUP | 145,864,965 | 145,864,994 | AAGTCTTAAGTTTCCCATAACCTGCCTGGA |
| TUR | 145,864,921 | 145,864,939 | TGCGGGCAGAGTTTCCTTG |
| TDF | 145,863,661 | 145,863,680 | TCAAACCTGCGCCAAAGAAG |
| TDP | 145,863,637 | 145,863,660 | TCTTGCAATACCTCGCGCGCTTTG |
| TDR | 145,863,588 | 145,863,606 | AGGTGGCTGACGAGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCACACTTTCAATACTGC |
| SD | GTGTGAAAGGGTACTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 255 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
