10829 Btg1 Status: Cancelled
| Gene | Btg1 |
| Chromosome | 10 |
| Deletion Start | 96,617,314 |
| Deletion End | 96,618,643 |
| Deletion Size | 1,330 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,617,392 | 96,617,411 | CCAAGTTCCTCCGCACCAAG |
| TUP | 96,617,418 | 96,617,439 | ACGAGCGAGCGACAGCTGCAGA |
| TUR | 96,617,440 | 96,617,457 | GCAGGCTCTGGCTGAAAG |
| TDF | 96,618,493 | 96,618,512 | CGTGCTGTACGAAGCCTCAC |
| TDP | 96,618,513 | 96,618,539 | CAGCAGGAGGTAGCACTCAAAACAGCA |
| TDR | 96,618,573 | 96,618,595 | GCCCAAGAGAAGTTCCTCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CCACTTCCTTTCCGCAGCC |
| SD | ACCACTCTATCTTGTGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 491 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 222 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
