10830 Capza3 Status: ES cell colonies screened / QC no positives
| Gene | Capza3 |
| Chromosome | 6 |
| Deletion Start | 140,041,661 |
| Deletion End | 140,042,622 |
| Deletion Size | 962 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,041,885 | 140,041,905 | TCCTCCTGTCTCACCACAATG |
| TUP | 140,041,906 | 140,041,931 | TGATGGGTGACTTCCGCTTCTTTGAC |
| TUR | 140,041,948 | 140,041,971 | TCTGGAGTAGGTCAAACCTGAAAG |
| TDF | 140,042,297 | 140,042,318 | GGACCTAAAGGAGAGCTTGGAG |
| TDP | 140,042,320 | 140,042,343 | TGGTCAATCAAGCCCAGTTGGCTC |
| TDR | 140,042,345 | 140,042,364 | CCACAAGCCTTGCGAAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTGAGTACTCATTGTGACGTC |
| SD | GACTTAATCATGGCCACCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 331 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
