10833 C1ql2 Status: Cancelled
| Gene | C1ql2 |
| Chromosome | 1 |
| Deletion Start | 120,341,103 |
| Deletion End | 120,342,646 |
| Deletion Size | 1,544 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,341,627 | 120,341,648 | AGGCTACGAGGTGCTCAAGTTC |
| TUP | 120,341,650 | 120,341,673 | ACGACGTGGTCACCAATCTTGGCA |
| TUR | 120,341,739 | 120,341,757 | CGCCACGCATGAGAATGTG |
| TDF | 120,342,014 | 120,342,032 | TGGGCGTTTGAGACTGGAC |
| TDP | 120,342,033 | 120,342,052 | CCCGGGTGCACAGTGACCCT |
| TDR | 120,342,087 | 120,342,106 | GCCACTTCAGGCCATGACTC |
PCR Primers
| Primer | Sequence |
| SU | GTCCGGATACCTCTCGTTG |
| SD | CGCCAGAGCTGGGATTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 294 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
