10834 C1ql4 Status: ES cell colonies screened / QC one positive
| Gene | C1ql4 |
| Chromosome | 15 |
| Deletion Start | 99,087,725 |
| Deletion End | 99,084,406 |
| Deletion Size | 3,320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,086,973 | 99,086,992 | GACAGGACAGTAGGCTGAGG |
| TUP | 99,086,944 | 99,086,971 | CAGAGCTAGAACTCAGGACTCAAAGGCC |
| TUR | 99,086,911 | 99,086,929 | GGCTACAGTCGGAGGTTGG |
| TDF | 99,084,817 | 99,084,837 | ATCAAGCTGGACGGTGGGAAG |
| TDP | 99,084,794 | 99,084,815 | TGCACGGCGGGAACACCAATAA |
| TDR | 99,084,754 | 99,084,776 | TCAGTCCGGGTAGATGATGAATC |
PCR Primers
| Primer | Sequence |
| SU | GAGATCGCCACCCTGACAG |
| SD | TGCTAGGAAGGAAGTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
