10837 Crygf Status: ES cell colonies screened / QC no positives
| Gene | Crygf |
| Chromosome | 1 |
| Deletion Start | 65,926,543 |
| Deletion End | 65,928,252 |
| Deletion Size | 1,710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,927,132 | 65,927,152 | GCTGAGAGTCCCTTTGGTGAG |
| TUP | 65,927,155 | 65,927,178 | CTGGTCTGCCGGCCAGTGTAAAGA |
| TUR | 65,927,240 | 65,927,259 | CCTGCCGGTAAGGTTCCATC |
| TDF | 65,927,960 | 65,927,980 | CGGACACTCAGTCCACTTCTC |
| TDP | 65,927,981 | 65,928,009 | ACAGGATCAGGATCTACGAGCGAGAGGAC |
| TDR | 65,928,012 | 65,928,030 | CTCCACCATCTGGCCTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCGAAAGCTCTGGAGTGTC |
| SD | ATCTGCTCAGCCCACAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 248 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
