10838 Crygs Status: ES cell colonies screened / QC no positives
| Gene | Crygs |
| Chromosome | 16 |
| Deletion Start | 22,811,352 |
| Deletion End | 22,805,262 |
| Deletion Size | 6,091 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,810,964 | 22,810,984 | GCCAGGCCAGTGAACTAAATC |
| TUP | 22,810,932 | 22,810,961 | ACAGCACTTCTGCATTCAAGTGGTTCTTGT |
| TUR | 22,810,888 | 22,810,908 | CGACAAGCAGTTGGGTCAAAC |
| TDF | 22,805,327 | 22,805,344 | AGTCGTTCCGCCGCATTG |
| TDP | 22,805,291 | 22,805,319 | ATGGCCTGGAGTGGGACCATAGAGTTCTC |
| TDR | 22,805,270 | 22,805,289 | CCCTCAAGTTCAGCCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTTGGGTGCCTTGATTCAGC |
| SD | GCTCCGTAAAGGGCAGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 383 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
