10839 Clec11a Status: Cancelled
| Gene | Clec11a |
| Chromosome | 7 |
| Deletion Start | 44,306,762 |
| Deletion End | 44,304,453 |
| Deletion Size | 2,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,306,316 | 44,306,339 | CCTTCTTCTAGTCCCAACCCTTTC |
| TUP | 44,306,288 | 44,306,312 | AGCCCTTCTCCCACACCAGAGGACA |
| TUR | 44,306,249 | 44,306,270 | CCACCCTGGCTTCTGAATACTC |
| TDF | 44,305,252 | 44,305,271 | GTCCTGGCTCTGCTTCATTG |
| TDP | 44,305,227 | 44,305,250 | TTTCCGGGCCTTAGCTCTTTGGCC |
| TDR | 44,305,200 | 44,305,217 | AGGTGGTGGCAGGCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCCTGGAAGCCAATAGG |
| SD | TTGCTTCCTCTGCTCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
