10840 Clec2d Status: ES cell colonies screened / QC no positives
| Gene | Clec2d |
| Chromosome | 6 |
| Deletion Start | 129,180,666 |
| Deletion End | 129,186,008 |
| Deletion Size | 5,343 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,180,983 | 129,181,003 | AGCAGCGAGTCACCTTCTAAC |
| TUP | 129,181,006 | 129,181,030 | CCAGAGCTGTAGCCACAGCGTTCTC |
| TUR | 129,181,039 | 129,181,062 | GGAAGAGTGGACTAGCAAGCTAAG |
| TDF | 129,185,030 | 129,185,051 | TGTGGCTCAGAGGTAGAGATTG |
| TDP | 129,185,052 | 129,185,077 | TGTTTACATGCAACAGCCAATCCCCA |
| TDR | 129,185,078 | 129,185,100 | GTTTGTGGGAATGTGGAGTTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACAGGAAAGTCAGTCTCCAG |
| SD | CTCTCCTATGTCCCTCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 391 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
