10841   Clec2h     Status: ES cell colonies screened / QC no positives

GeneClec2h
Chromosome6
Deletion Start128,662,445
Deletion End128,675,932
Deletion Size13,488
Genome Build38
MGI IDMGI:2136934
NCBI Gene ID94071
BAC ID347J5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,662,567128,662,591GGGATAGAGGGAAGTTTCAACAGAG
TUP128,662,596128,662,618TCAGAGGAGCTGAGTGTCCTGCC
TUR128,662,650128,662,668TGCCTCCAAACATCACCAC
TDF128,675,234128,675,252CAGCAAGCCAGTGCAGAAC
TDP128,675,253128,675,281TGCATGGGTAAGACTTGTTGGTTCTCTGT
TDR128,675,287128,675,310CTTTGCTTCAGTGAACAGGTACAG

PCR Primers

PrimerSequence
SUCCTACCTTGTGATTCTGTTTC
SDCTACTCAGGTTGTCCGGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev438769 bp-++
TUFTUR102 bp++-
TDFTDR77 bp++-
NeoFwdSD267 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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