10842 Clec3a Status: Cancelled
| Gene | Clec3a |
| Chromosome | 8 |
| Deletion Start | 114,418,089 |
| Deletion End | 114,425,868 |
| Deletion Size | 7,798 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,418,113 | 114,418,133 | ATCCTGGTGGTCAGCTTACTC |
| TUP | 114,418,135 | 114,418,155 | TGGACCAGACCGACGGCTACC |
| TUR | 114,418,166 | 114,418,185 | TGCTGTGTTTCCTGGCTTTC |
| TDF | 114,424,941 | 114,424,960 | GAGTCACGGCAAGCATTCTG |
| TDP | 114,424,961 | 114,424,982 | TCTCCGCCTCTCAAGTGCTCCA |
| TDR | 114,425,005 | 114,425,024 | GAGCAAACACAAGCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | AGCGTGAGGTGTAAGAACAG |
| SD | CTTTCTTACCATGTCTGGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 289 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
