10847 Cyb561d2 Status: ES cell colonies screened / QC no positives
| Gene | Cyb561d2 |
| Chromosome | 9 |
| Deletion Start | 107,541,620 |
| Deletion End | 107,539,861 |
| Deletion Size | 1,760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,540,944 | 107,540,962 | GGCGTTGTTGGTGGCTCTC |
| TUP | 107,540,909 | 107,540,938 | CCTTGTAGGCATTTAGTGCCAGTGTAGACG |
| TUR | 107,540,882 | 107,540,904 | CAGAAGCAGGAGTGAGGACATAC |
| TDF | 107,540,241 | 107,540,260 | TCTGCTGGGTCTAGGTCTTG |
| TDP | 107,540,215 | 107,540,240 | TCATCCTCCACAAAGAGCAGCTTGGC |
| TDR | 107,540,191 | 107,540,210 | ATGCCGAGTGGTCAGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCATTCTGGGATTTGTAGTC |
| SD | CTTTCCTCCATGATTTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
