10848 Naa15 Status: Cancelled
| Gene | Naa15 |
| Chromosome | 3 |
| Deletion Start | 51,472,797 |
| Deletion End | 51,445,178 |
| Deletion Size | 27,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,416,762 | 51,416,780 | TCGGAAGGAACAGGTGGTG |
| TUP | 51,416,781 | 51,416,802 | TGGCCGAGGCAGGGACTCATTC |
| TUR | 51,416,810 | 51,416,830 | CGGAGAGGGAGAAGTGCTTTC |
| TDF | 51,443,490 | 51,443,511 | CAACCAGCTCATCTGTCAGCAG |
| TDP | 51,443,518 | 51,443,546 | CCACTGGCCTTTAGTATTCATTGTCTGGC |
| TDR | 51,443,570 | 51,443,591 | TGGAGCCAGAGAATTGGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGTTACCACGTGAACCGC |
| SD | GAGAATAAGGACCACTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
