| Gene | Otol1 |
| Chromosome | 3 |
| Deletion Start | 70,028,129 |
| Deletion End | 70,018,497 |
| Deletion Size | 9,633 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,019,165 | 70,019,186 | TCAGAAGTCGGAAGAAGGTGTC |
| TUP | 70,019,190 | 70,019,217 | TCTTCTGGCACTGGAGTTAATTGCTGAC |
| TUR | 70,019,219 | 70,019,241 | CCAACATCCACAAGGTAGCTCAC |
| TDF | 70,028,006 | 70,028,026 | GATCAAGTGTGGCTGGAAGTG |
| TDP | 70,028,027 | 70,028,054 | TCAAAGGACTGGAATGGGCTGTATGTCG |
| TDR | 70,028,083 | 70,028,103 | TCCTCTGGGTACAGAAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTGAGTGAGGTCAGGGAGG |
| SD | CATAAGTCTCATTCATAGCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10850C-G6 | VGB6 | C57BL/6NTac | |
