10859 AI314180 Status: Cancelled
| Gene | AI314180 |
| Chromosome | 4 |
| Deletion Start | 58,912,494 |
| Deletion End | 58,885,372 |
| Deletion Size | 27,123 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,911,655 | 58,911,672 | TCGTAGGCCGCAGGTTTG |
| TUP | 58,911,623 | 58,911,651 | TGTCACGTCGTGAAATTGAAACCAGAGCC |
| TUR | 58,911,586 | 58,911,606 | GCCGAAGCAGATTTCGAGCAG |
| TDF | 58,885,963 | 58,885,983 | TGTCCAACATGGTTCTCACTC |
| TDP | 58,885,932 | 58,885,957 | TCCCTGTGGGATACTTGTCTCGGTCC |
| TDR | 58,885,873 | 58,885,897 | GTCAGGGACTAACTGATGAAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGTCCCTCTGGGCTTTAAC |
| SD | ATAGCAGCTGTACTAGGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
