10862 Fem1a Status: Cancelled
| Gene | Fem1a |
| Chromosome | 17 |
| Deletion Start | 56,256,894 |
| Deletion End | 56,258,911 |
| Deletion Size | 2,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,257,697 | 56,257,717 | CACCCCAGAAGAAGCAGAACC |
| TUP | 56,257,720 | 56,257,742 | ATGAGAGCTGCTGCCCTACCAGC |
| TUR | 56,257,796 | 56,257,816 | GCTCCAAGCAGATCCCTTTTC |
| TDF | 56,258,798 | 56,258,816 | TGCCCTGGACAGGAACAAG |
| TDP | 56,258,822 | 56,258,850 | TTATAAGGGCTTCATCCCAGAGGAGCTGG |
| TDR | 56,258,862 | 56,258,881 | GGCTAGGCTCAATGCAACTG |
PCR Primers
| Primer | Sequence |
| SU | CTAACCATTGGCCTTGCTTG |
| SD | AACATCTAGGTGGTCAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
