10865   Fgf21     Status: ES cell clone microinjected

GeneFgf21
Chromosome7
Deletion Start45,615,303
Deletion End45,614,002
Deletion Size1,302
Genome Build38
MGI IDMGI:1861377
NCBI Gene ID56636
BAC ID223F11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF45,615,13845,615,160CCTCTACACAGATGACGACCAAG
TUP45,615,11245,615,137ACACTGAAGCCCACCTGGAGATCAGG
TUR45,615,08845,615,105TGCGCCTACCACTGTTCC
TDF45,614,60845,614,632GTACCCAGGACCAATAAATGACAAG
TDP45,614,57845,614,599TGGTGGGCAGAATGCCAACCTA
TDR45,614,53645,614,554AGCAGGTTCAGGCCCAGTG

PCR Primers

PrimerSequence
SUTCTGGTATTTCTGCGTTCAC
SDGTACCAGGAATGCTTTATCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev321 bp-++
TUFTUR73 bp++-
TDFTDR97 bp++-
NeoFwdSD314 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++