10865 Fgf21 Status: ES cell clone microinjected
Gene | Fgf21 |
Chromosome | 7 |
Deletion Start | 45,615,303 |
Deletion End | 45,614,002 |
Deletion Size | 1,302 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 45,615,138 | 45,615,160 | CCTCTACACAGATGACGACCAAG |
TUP | 45,615,112 | 45,615,137 | ACACTGAAGCCCACCTGGAGATCAGG |
TUR | 45,615,088 | 45,615,105 | TGCGCCTACCACTGTTCC |
TDF | 45,614,608 | 45,614,632 | GTACCCAGGACCAATAAATGACAAG |
TDP | 45,614,578 | 45,614,599 | TGGTGGGCAGAATGCCAACCTA |
TDR | 45,614,536 | 45,614,554 | AGCAGGTTCAGGCCCAGTG |
PCR Primers
Primer | Sequence |
SU | TCTGGTATTTCTGCGTTCAC |
SD | GTACCAGGAATGCTTTATCAG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 321 bp | - | + | + |
TUF | TUR | 73 bp | + | + | - |
TDF | TDR | 97 bp | + | + | - |
NeoFwd | SD | 314 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |