10865 Fgf21 Status: ES cell clone microinjected
| Gene | Fgf21 |
| Chromosome | 7 |
| Deletion Start | 45,615,303 |
| Deletion End | 45,614,002 |
| Deletion Size | 1,302 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,615,138 | 45,615,160 | CCTCTACACAGATGACGACCAAG |
| TUP | 45,615,112 | 45,615,137 | ACACTGAAGCCCACCTGGAGATCAGG |
| TUR | 45,615,088 | 45,615,105 | TGCGCCTACCACTGTTCC |
| TDF | 45,614,608 | 45,614,632 | GTACCCAGGACCAATAAATGACAAG |
| TDP | 45,614,578 | 45,614,599 | TGGTGGGCAGAATGCCAACCTA |
| TDR | 45,614,536 | 45,614,554 | AGCAGGTTCAGGCCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGTATTTCTGCGTTCAC |
| SD | GTACCAGGAATGCTTTATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
