10867 Hapln3 Status: ES cell colonies screened / QC no positives
| Gene | Hapln3 |
| Chromosome | 7 |
| Deletion Start | 79,123,490 |
| Deletion End | 79,117,027 |
| Deletion Size | 6,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,122,983 | 79,123,007 | GTTCTGTTGCTTAGGAAGTTCTGTC |
| TUP | 79,122,958 | 79,122,981 | TTTGGACCCTGAGCTCTGACAGCC |
| TUR | 79,122,915 | 79,122,933 | CAGGGCTCCTCCCAAAGAC |
| TDF | 79,117,507 | 79,117,529 | TGCTGGATAGCACTTAGATGGTC |
| TDP | 79,117,484 | 79,117,506 | CACATTCCCACAGGGCGGGTGTA |
| TDR | 79,117,452 | 79,117,469 | GCAGCGTCAGCTTCTTGG |
PCR Primers
| Primer | Sequence |
| SU | TTGAAACAAACTTTCCAGTGC |
| SD | ACACACAGCCAGAGAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 491 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 425 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
