| Gene | Hddc3 |
| Chromosome | 7 |
| Deletion Start | 80,343,122 |
| Deletion End | 80,345,779 |
| Deletion Size | 2,658 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,343,789 | 80,343,808 | AGGTTCGACGCTTGGTGGAG |
| TUP | 80,343,810 | 80,343,839 | AGGTAACAGATGACAAGACTCTGCCCAAAC |
| TUR | 80,343,842 | 80,343,862 | CACCTGTTGCCGCTTTCTTTC |
| TDF | 80,345,515 | 80,345,534 | GCTGGGTCATGGAGAAGTTG |
| TDP | 80,345,535 | 80,345,560 | TCAGATCATGCCATCGCAACTCACGG |
| TDR | 80,345,569 | 80,345,587 | TGTTTCCTGGCAGGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGATTATTGCAATAGCC |
| SD | AAACCACGGACGCTTATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 457 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 368 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10868B-A7 | VGB6 | C57BL/6NTac | |
| 10868B-E12 | VGB6 | C57BL/6NTac | |
| 10868B-G12 | VGB6 | C57BL/6NTac | |
