10876 Tex15 Status: ES cell colonies screened / QC no positives
| Gene | Tex15 |
| Chromosome | 8 |
| Deletion Start | 33,570,547 |
| Deletion End | 33,585,544 |
| Deletion Size | 14,998 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,571,259 | 33,571,277 | GCTCAAAGGGCCATGACTG |
| TUP | 33,571,281 | 33,571,310 | AGCATCCAGTAGCATTGCTGTTACAGAACA |
| TUR | 33,571,318 | 33,571,339 | GGAAGGAAGAACTGTGTTGCTC |
| TDF | 33,584,643 | 33,584,665 | AACCTTGCAGTTCCCTATTGGAG |
| TDP | 33,584,667 | 33,584,689 | TCCCACCACTGCTCAGTGAGGTA |
| TDR | 33,584,705 | 33,584,722 | TGGACCAAGGGCACTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGGCTAAAATGTTCAGTC |
| SD | AAGATTTCTGTCATTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 410 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 388 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
