10877 Fam187b Status: Cancelled
| Gene | Fam187b |
| Chromosome | 7 |
| Deletion Start | 30,977,071 |
| Deletion End | 30,989,343 |
| Deletion Size | 12,273 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,977,712 | 30,977,734 | TGACACATATCTGCTGGACAAAC |
| TUP | 30,977,739 | 30,977,763 | CAGCAATGCTCGACTCAAGTGTCCC |
| TUR | 30,977,780 | 30,977,797 | CTGCGCCTGCTCATTACC |
| TDF | 30,989,197 | 30,989,214 | CAGCCAGACAAGGCAGAC |
| TDP | 30,989,216 | 30,989,245 | CCGTTCTTAGGAGATTGAAGTTGATGGTGC |
| TDR | 30,989,253 | 30,989,271 | TCCAACGGCCAGTACAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCCAGGTTGTTATATTTG |
| SD | GAGCAGGGCACAGAACAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 274 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 265 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
