10881   Usp17l5     Status: ES cell colonies screened / QC no positives

GeneUsp17l5
Chromosome7
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:107698
NCBI Gene ID13532
BAC ID69K19
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCGAGATGCAATCACTGTGTGGA
TUPTAGCTAGGGCACTACCATGAGCCAC
TURCCCTCTGCTTGCTGCTTAC
TDFTCTCTTATGTCAAAGCCAGACATG
TDPTGGATGATACTAAGGTCACCAGCTGCGA
TDRCCTGTTTGAGGTCAGTCTGCTG

PCR Primers

PrimerSequence
SUGAGCTAAATTCAGGGAAATG
SDTTGCAGGAGAGGCTATGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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