10884 Stbd1 Status: ES cell colonies screened / QC no positives
| Gene | Stbd1 |
| Chromosome | 5 |
| Deletion Start | 92,603,093 |
| Deletion End | 92,605,673 |
| Deletion Size | 2,581 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,603,807 | 92,603,825 | GCTGGCGTGTGAACAAGTG |
| TUP | 92,603,827 | 92,603,855 | TGGCCTACTAGGGAATCGAACTCTGATGA |
| TUR | 92,603,888 | 92,603,906 | TTGCCTGATGCAGGGTCTC |
| TDF | 92,605,506 | 92,605,528 | GGCTCTGGTCTCATTCTGTCTTC |
| TDP | 92,605,529 | 92,605,551 | CTGCCTGCAGACACAGTGGTGGA |
| TDR | 92,605,583 | 92,605,604 | TGCATTCTTCCCAACGAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | GGGCCAGGAAAATTCGCGGC |
| SD | TACCCAGGATGATCAATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 261 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
