| Gene | Kcnk18 |
| Chromosome | 19 |
| Deletion Start | 59,219,717 |
| Deletion End | 59,235,665 |
| Deletion Size | 15,949 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,219,923 | 59,219,946 | GAGGAGAACCCTGAGTTGAAGAAG |
| TUP | 59,219,948 | 59,219,971 | TCCTGGACGATCTGTGCAACATCC |
| TUR | 59,220,007 | 59,220,025 | GCACCTCCGAGGCAGTAAC |
| TDF | 59,235,015 | 59,235,034 | GAAGCAGCCTGACAGCAAAC |
| TDP | 59,235,035 | 59,235,056 | CGGTGGAGGAAGCCATCCCTCA |
| TDR | 59,235,057 | 59,235,078 | CACACCAGCATCAATGACAATC |
PCR Primers
| Primer | Sequence |
| SU | GGAAAGGCAGAGCAAAGTAC |
| SD | AGCAGTGACATCACTAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | 325 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10886B-F10 | VGB6 | C57BL/6NTac | |
| 10886B-F11 | VGB6 | C57BL/6NTac | |
| 10886A-B9 | VGB6 | C57BL/6NTac | |
