10889 Dynlt3 Status: ES cell colonies screened / QC no positives
| Gene | Dynlt3 |
| Chromosome | X |
| Deletion Start | 9,662,895 |
| Deletion End | 9,655,886 |
| Deletion Size | 7,010 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,662,467 | 9,662,485 | CCCTTTGGGCCTCCTTGTG |
| TUP | 9,662,440 | 9,662,462 | TGCTGCAAGGTGGCTCTAGTCCC |
| TUR | 9,662,406 | 9,662,427 | GCACTTCCTCTAGCCTTTCCTG |
| TDF | 9,656,825 | 9,656,845 | TGAACCGCTTGCCCTTTGTAG |
| TDP | 9,656,797 | 9,656,821 | CAGTTGGCTTTGCCTACTGAGACCC |
| TDR | 9,656,763 | 9,656,781 | AGGCACTGATGGCCGAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCGCTTGCTGGATAACTGTG |
| SD | TTTCTCTGCATTTCTCCTTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
