10890 Chml Status: ES cell colonies screened / QC no positives
| Gene | Chml |
| Chromosome | 1 |
| Deletion Start | 175,688,350 |
| Deletion End | 175,686,444 |
| Deletion Size | 1,907 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 175,688,202 | 175,688,223 | GAGGGAATTGGGCGAGTTTCAG |
| TUP | 175,688,173 | 175,688,197 | ACAGGTTTGCAGTCCTGGCTGAAGG |
| TUR | 175,688,145 | 175,688,164 | TCCTCGCTGTCATGGTTCTG |
| TDF | 175,686,635 | 175,686,656 | CTGTGGCTTACCTTCCAATGTG |
| TDP | 175,686,611 | 175,686,633 | ACATCTGCTCTGGGCCTGACTGG |
| TDR | 175,686,573 | 175,686,591 | GTTTCCGCCTGCTTGACTG |
PCR Primers
| Primer | Sequence |
| SU | AGTCAGGCTCCAGTGGATTG |
| SD | ACAGTAGCGGCAGCAGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
