10891 Spz1 Status: Cancelled
| Gene | Spz1 |
| Chromosome | 13 |
| Deletion Start | 92,575,963 |
| Deletion End | 92,574,739 |
| Deletion Size | 1,225 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,575,913 | 92,575,932 | CCCGGTGTCCATCTCCTAAC |
| TUP | 92,575,886 | 92,575,911 | CTGCTCCTGGTGCTAAGCAAGAACCA |
| TUR | 92,575,859 | 92,575,880 | GAGGGAAATGGTGATCCCAGAG |
| TDF | 92,574,890 | 92,574,910 | GCTCGGAATAACCGCTTCAAC |
| TDP | 92,574,861 | 92,574,888 | CCCGTGTTGCAAAGAAATCTCTGGTGGG |
| TDR | 92,574,812 | 92,574,833 | ATCTGCTTCTGGCGACTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAATAAAGACCTAGTAGC |
| SD | CTAAATACTAATTTGAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 393 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
