10894 1100001G20Rik Status: ES cell colonies screened / QC no positives
| Gene | 1100001G20Rik |
| Chromosome | 11 |
| Deletion Start | 83,747,030 |
| Deletion End | 83,749,354 |
| Deletion Size | 2,325 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,747,533 | 83,747,554 | GGCCAGGAACTGACTGTGTAAC |
| TUP | 83,747,556 | 83,747,579 | CAGGCTGACCTCAACAACATGGCA |
| TUR | 83,747,582 | 83,747,606 | CAGCATAGCAAGATGAAGCAGGAAG |
| TDF | 83,749,196 | 83,749,216 | CACCATGCCTGCTTTCAACTG |
| TDP | 83,749,223 | 83,749,248 | TCTGACATAGGCACCTGTGCTGAGCT |
| TDR | 83,749,280 | 83,749,304 | TGCATCCAATACTGACACATTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATTGAAAAGGAACGAGAGTC |
| SD | AGAAATCAAATAGGCACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 423 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
