10894 1100001G20Rik Status: ES cell colonies screened / QC no positives
| Gene | 1100001G20Rik |
| Chromosome | 11 |
| Deletion Start | 83,560,532 |
| Deletion End | 83,562,856 |
| Deletion Size | 2,325 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCCAGGAACTGACTGTGTAAC |
| TUP | | | CAGGCTGACCTCAACAACATGGCA |
| TUR | | | CAGCATAGCAAGATGAAGCAGGAAG |
| TDF | | | CACCATGCCTGCTTTCAACTG |
| TDP | | | TCTGACATAGGCACCTGTGCTGAGCT
|
| TDR | | | TGCATCCAATACTGACACATTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATTGAAAAGGAACGAGAGTC |
| SD | AGAAATCAAATAGGCACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| NeoFwd | SD | 423 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
