10895 Rnase6 Status: Cancelled
| Gene | Rnase6 |
| Chromosome | 14 |
| Deletion Start | 51,130,150 |
| Deletion End | 51,130,611 |
| Deletion Size | 481 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,130,271 | 51,130,294 | AGCACGTACAGACAAGTCGTCAAC |
| TUP | 51,130,296 | 51,130,318 | ATGCAACACAGCCATGCGCGGTG |
| TUR | 51,130,353 | 51,130,376 | TGGAAAGATTCATGCAGAAAGGTG |
| TDF | 51,130,393 | 51,130,413 | TGCAGTTTGCACAATATCACC |
| TDP | 51,130,418 | 51,130,442 | AGAACGGTCGGAAGAACTGCCACGA |
| TDR | 51,130,453 | 51,130,474 | AGCAGTCAGTCATCTTGACAGG |
PCR Primers
| Primer | Sequence |
| SU | CCCAACAGGAAGCATTCATG |
| SD | ACCTCTGTGCACTAAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
