10897 Apba2 Status: Cancelled
| Gene | Apba2 |
| Chromosome | 7 |
| Deletion Start | 64,695,048 |
| Deletion End | 64,715,536 |
| Deletion Size | 20,489 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,695,362 | 64,695,381 | ACATCCGCTACTGTCCTGAG |
| TUP | 64,695,386 | 64,695,411 | ACAGCTACCTGGAGGGCATGGACTGT |
| TUR | 64,695,421 | 64,695,441 | TGCACCGTGTGCTATGTATTC |
| TDF | 64,714,880 | 64,714,899 | GCTCCCGATCTGGTAGAAAC |
| TDP | 64,714,901 | 64,714,930 | CAAGGTTAGCCTGGATTTCTCAAGAGCAGA |
| TDR | 64,714,931 | 64,714,953 | GAACCACCCTAAATGACAATGAC |
PCR Primers
| Primer | Sequence |
| SU | ATATATCTCATATCAGATAC |
| SD | CTGTGATGCAGTTAATGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
