10902   Klhl34     Status: ES cell colonies screened / QC positives

GeneKlhl34
ChromosomeX
Deletion Start157,818,438
Deletion End157,820,370
Deletion Size1,933
Genome Build38
MGI IDMGI:2685234
NCBI Gene ID245683
BAC ID284L9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF157,819,192157,819,213TCCAGGCCCTCAATTACCATAC
TUP157,819,214157,819,235AGCACCCTCCAGCCAGCCGCTT
TUR157,819,276157,819,299TGAGACCACCAACTAGCAAGATAC
TDF157,819,626157,819,643TATGACCCGCGCTTCCAC
TDP157,819,644157,819,668ACTTGGACACAGGTGCCTGCCATGC
TDR157,819,682157,819,702ACCACACCACACCAGAAGTAG

PCR Primers

PrimerSequence
SUGGAGGCGGCTTTGGGCAACG
SDCACAGGTACTTGAAAAACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev449 bp-++
TUFTUR108 bp++-
TDFTDR77 bp++-
NeoFwdSD417 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10902A-C3VGB6C57BL/6NTac
10902A-D3VGB6C57BL/6NTac
10902A-D12VGB6C57BL/6NTac
10902A-E2VGB6C57BL/6NTac
10902A-H12VGB6C57BL/6NTac
10902A-G4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image