10910 Wfdc10 Status: Cancelled
| Gene | Wfdc10 |
| Chromosome | 2 |
| Deletion Start | 164,656,092 |
| Deletion End | 164,657,351 |
| Deletion Size | 1,260 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,656,128 | 164,656,149 | GCTCTTCCTTGGTGTGCTGTTG |
| TUP | 164,656,151 | 164,656,172 | TGCTTTCCCTAGCCGACGGAGG |
| TUR | 164,656,210 | 164,656,232 | GCTACCCACAAACCCTACTCATC |
| TDF | 164,657,112 | 164,657,129 | TCTGTGCAGCCGCCATTG |
| TDP | 164,657,130 | 164,657,151 | TGAAGCCCACCAAGACTGCCAA |
| TDR | 164,657,153 | 164,657,176 | CAGCGGAGCAGCAGATGTTATTTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGCTCACAGACATTTGC |
| SD | ACTCCCACCCTCTACCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
