10917 Mocs3 Status: Cancelled
| Gene | Mocs3 |
| Chromosome | 2 |
| Deletion Start | 168,230,620 |
| Deletion End | 168,232,173 |
| Deletion Size | 1,554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 168,231,163 | 168,231,180 | GCAGACTGCTGCGACAAC |
| TUP | 168,231,184 | 168,231,205 | CCCACGCGCTACCTGGTGAACG |
| TUR | 168,231,268 | 168,231,287 | TCGTGGTGGTAGACGGTCAT |
| TDF | 168,231,779 | 168,231,798 | ATGCGGACAGCCTGAAACTC |
| TDP | 168,231,806 | 168,231,828 | CTGCCCTCCGGAAAGGGAAGCAG |
| TDR | 168,231,848 | 168,231,870 | GCAAATCACATACACCGGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGACGTCCCAGTGTGGT |
| SD | GTAGCCCGAGTTAAGCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 311 bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
