10921 Defb50 Status: Cancelled
| Gene | Defb50 |
| Chromosome | 8 |
| Deletion Start | 21,823,595 |
| Deletion End | 21,831,294 |
| Deletion Size | 7,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,823,875 | 21,823,896 | TCTCAGCAGCTCATCATTTGTC |
| TUP | 21,823,899 | 21,823,923 | TGCCATGAGCATGCAGGAACTATCC |
| TUR | 21,823,954 | 21,823,976 | GCGAAACAGTGGCATTTCACTTG |
| TDF | 21,830,329 | 21,830,351 | CACCAGTGACATACTTCCTCCAA |
| TDP | 21,830,367 | 21,830,394 | TCCCCAAAAGGAGCTATCATAGAGACCA |
| TDR | 21,830,453 | 21,830,472 | TGAGCTACCACCTTGCTTCA |
PCR Primers
| Primer | Sequence |
| SU | CACAAACTCCAGCTGCAGAC |
| SD | TCTCTTGAGTCAACTCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 144 bp | + | + | - |
| NeoFwd | SD | 285 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
