10922 Defb12 Status: ES cell colonies screened / QC positives
| Gene | Defb12 |
| Chromosome | 8 |
| Deletion Start | 19,114,817 |
| Deletion End | 19,112,652 |
| Deletion Size | 2,166 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGTACACTGGTCTTTCTGGCTTC |
| TUP | | | CAGGAGTGCTGGTCTTGAGAAACCCA |
| TUR | | | ATGGCTTTCGAGGGACTACTGATG |
| TDF | | | ACTCGGCTAGGCTGACCAC |
| TDP | | | TGAGAGTCAGCTGTCCCTGCTTCC
|
| TDR | | | TTGTGTCACTCACGGTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGTAGAAGTGGGCACAGC |
| SD | GTTCTGGAGTTGTGCAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
