10924 Gja6 Status: Cancelled
| Gene | Gja6 |
| Chromosome | X |
| Deletion Start | 160,903,931 |
| Deletion End | 160,903,033 |
| Deletion Size | 899 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 160,903,853 | 160,903,875 | TGGAGGAAAGGTATGGATCAAGG |
| TUP | 160,903,821 | 160,903,842 | TTTCCGCATCCTGCTCCTGGGC |
| TUR | 160,903,778 | 160,903,798 | GGAACTCAAACTGCTCGTCAC |
| TDF | 160,903,447 | 160,903,467 | GGCCAGCATCTTCTTCAAGTC |
| TDP | 160,903,417 | 160,903,440 | CGAGATGGCCTTCCTCCTGATCCA |
| TDR | 160,903,372 | 160,903,394 | TCACAGATGTAAAGGGCACTCAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCTTTCACCTGGTGTCAG |
| SD | GCTTTCACAATGCTTTCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 323 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 311 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
