10926 Ift57 Status: Cancelled
| Gene | Ift57 |
| Chromosome | 16 |
| Deletion Start | 49,699,341 |
| Deletion End | 49,711,901 |
| Deletion Size | 12,578 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,700,063 | 49,700,084 | TGGGAAGTGGAGAAGGCAAATG |
| TUP | 49,700,092 | 49,700,119 | TGTTTGGAATGGCCCTCAATATAGGACC |
| TUR | 49,700,124 | 49,700,141 | ACTACCGCAAGCGGGAAC |
| TDF | 49,711,245 | 49,711,266 | GTGAAACGTGCATCTTCAGTTC |
| TDP | 49,711,267 | 49,711,293 | TGGAGAAATGGCTCAGTGGCTAAAACC |
| TDR | 49,711,316 | 49,711,336 | GGAGCCAGAAAGCTGGATGTC |
PCR Primers
| Primer | Sequence |
| SU | ATGCAGCGCTAAATAGCTGG |
| SD | ATTCCTGGTCTACTTCAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 465 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 387 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
